在转录的不同阶段,RNA聚合酶II酸化的独特相互作用体在转录的不同阶段发生
Rosamaria Y Moreno1, Kyle J Juetten2, Svetlana B Panina1
1Department of Molecular Biosciences, University of Texas, Austin, TX, USA.
iScience
|September 4, 2023
概括
的RNA聚合酶II.
科学领域:
- 分子生物学分子生物学
- 基因表达 基因表达
- 生物化学 生物化学
背景情况:
- 细胞转录涉及RNA聚合酶II的C终端域 (CTD) 上的动态后翻译修饰 (PTM).
- CTD酸化模式,特别是Ser5和Ser2的酸化模式,在空间和时间上与不同的转录阶段相关.
- 这些酸化事件将特定的转录调节剂招募到RNA聚合酶II.
研究的目的:
- 为了研究不同转录阶段与RNA聚合酶II相关的蛋白质相互作用体.
- 了解特定的CTD酸化模式 (Ser2和Ser5) 如何决定蛋白质合作伙伴的招募.
- 为了确定参与转录相关蛋白质招募的新型调节者.
主要方法:
- 在 Ser5 和 Ser2 *in vitro* 中重建CTD酸化模式.
- 在定义的酸化状态下,对RNA聚合酶II招募的蛋白质相互作用的分析.
- 特定的蛋白质-PTM相互作用的特征,包括平衡内网膜蛋白 (CHERP) 的作用.
主要成果:
- 基于CTD Ser2和Ser5酸化,不同的蛋白质相互作用被招募到RNA聚合酶II中.
- 鉴定出稳态内细胞网膜蛋白 (CHERP) 是一种与-Ser2 CTD heptad 结合的蛋白质.
- 波尔II与CHERP的关联招募了一个辅助拼接综合体,影响了替代拼接事件.
结论:
- 在转录过程中,CTD酸化模式作为代码来招募特定阶段的蛋白质相互作用体.
- 通过与-Ser2 CTD的相互作用,CHERP在协调转录和替代拼接方面发挥着作用.
- 这项研究阐明了PTM编码的招募机制,该机制控制着转录调节.
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