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在肺腺癌中RRM2-介导的Wnt/β-catenin信号通路激活:潜在的预后生物标志物

Yongjie Jiang¹, Xing Hu¹, Min Pang¹

¹Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.

Oncology letters

|September 4, 2023

概括

核酸减少酶M2 (RRM2) 在肺腺癌中高度表达,导致癌症的进展和对西斯普拉丁的耐药性. 向RRM2可能为肺腺癌提供新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

肺腺癌是全球癌症相关死亡的主要原因之一.
确定新的治疗点和了解耐药性机制对于改善患者的治疗结果至关重要.

研究的目的:

在肺腺癌中研究核核酸还原酶M2 (RRM2) 的作用和机制.
评估RRM2作为肺腺癌的潜在治疗标和生物标记物.

主要方法:

对癌症基因组图谱 (TCGA) 数据库对RRM2表达和临床相关性的分析.
西部斑块和RT-qPCR用于评估肺腺癌细胞系 (A549和A549/DDP) 中的RRM2表达.
细胞检测包括CCK-8,殖民地形成,Transwell迁移,细胞周期和细胞亡分析,以确定RRM2功能及其在西斯普拉丁耐药性中的作用.
研究Wnt/β-catenin信号通路的参与.

主要成果:

在肺腺癌组织中,RRM2显著上调,与晚期TMN阶段相关.
在耐思的细胞 (A549/DDP) 中,RRM2的表达更高,并且由思治疗诱导.
RRM2倒置减少了细胞增殖,迁移,并诱导了细胞亡和S相细胞循环停止.
在A549和A549/DDP细胞中,通过Wnt/β-catenin通路介导,对cisplatin的敏感性增加.
免疫细胞透分析揭示了基于RRM2表达水平的独特模式.

结论:

RRM2在肺腺癌的进展和对西斯普拉丁的耐药性方面发挥着至关重要的作用.
通过Wnt/β-catenin信号通路,RRM2影响肺腺癌的生长和化学抵抗.
在肺腺癌治疗中,RRM2 是一个有前途的治疗标.

关键词:

在Wnt/β-catenin信号通路中.灭症 (apoptosis) 是一种死亡的过程.细胞循环中的细胞循环.肺部腺癌的癌症.核糖核酸减少酶 M2 的作用.

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