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长期给予青春期老鼠的荷尔蒙和非荷尔蒙口服避孕药对骨质,质量和新陈代谢有不同的影响
Konica Porwal1, Shivani Sharma1,2, Saroj Kumar3
1Division of Endocrinology and Centre for Research in Anabolic Skeletal Targets in Health and Illness (ASTHI), Council of Scientific & Industrial Research-Central Drug Research Institute, Lucknow, India.
激素和非激素避孕药都会对大鼠的骨健康产生负面影响,组合激素避孕药显示骨强度减少较大. 对生物标志物的进一步研究是有必要的.
科学领域:
- 内分泌学和生殖科学 生殖科学
- 骨生物学和新陈代谢
- 药理学 药理学是指药理学的学科.
背景情况:
- 口服避孕药 (OCs) 被生育年龄的女性广泛使用.
- 了解荷尔蒙和非荷尔蒙OCs对骨的长期影响对于妇女健康至关重要.
- 之前的研究已经产生了关于OCs对骨矿物质密度的影响的混合结果.
研究的目的:
- 研究组合激素避孕药 (CHC) 和非激素避孕药 (NHC) 对青春期雌性大鼠的骨质,矿化,组成,机械性质和代谢物的影响.
- 为了比较CHC与NHC对骨的影响.
- 为了确定OC疗效和安全的潜在生物标志物.
主要方法:
- 处于青春期的雌性SD大鼠被给予CHC (乙inyl Estradiol和孕激素) 或NHC (Ormeloxifene) 3个月和7个月,剂量相当于人类.
- 使用MicroCT评估了骨微架构和BMD.
- 通过静态和动态组织形态测量来评估骨的形成和矿化.
- 骨强度和材料组成通过三点曲试验,纳米缩,FTIR和循环参考点缩 (cRPI) 进行测量.
- 分析了骨和血清代谢组.
主要成果:
- 7个月后,CHC和NHC都减少了骨质量,并在大腿骨和腰椎中恶化了状小架构.
- 这两种OC都降低了矿物质:矩阵比,增加了非矿物质矩阵,表明骨质受损.
- 在静态负荷下,CHC显著降低了骨强度,而NHC的影响较小.
- 与NHC相比,CHC改变了更多的骨和血清中的代谢途径,其中6种常见代谢物被确定为潜在的生物标志物.
结论:
- 激素和非激素口服避孕药都对大鼠的骨参数产生不良影响.
- 与非激素避孕药相比,组合激素避孕药对骨强度产生更大的负面影响.
- 代谢分析揭示了与CHC使用相关的潜在血清和骨生物标志物,为骨健康监测提供了洞察力.
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