葡萄糖失调通过调节自和YAP1/TAZ表达来促进人类膀癌的瘤发生
Shi Li1, Banzhan Ruan2, Zhi Wang3
1Department of Urology, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
高血糖水平会损害自并促进2型糖尿病 (T2D) 患者膀癌 (BC) 的进展. 在这个过程中,YAP1/TAZ/AMPK通路是关键的,它为T2D的BC提供了潜在的治疗标和预后标志物.
科学领域:
- 在瘤学瘤学.
- 代谢疾病 代谢疾病
- 细胞生物学 细胞生物学
背景情况:
- 葡萄糖失调与癌症的发展有关,特别是在2型糖尿病 (T2D) 患者中.
- 膀癌 (BC) 的患病率在T2D患者中较高,需要对潜在机制进行调查.
- 自在细胞对代谢压力和癌症进展的反应中起着关键作用.
研究的目的:
- 在葡萄糖失调条件下,阐明人类膀癌 (BC) 中自调节的机制.
- 调查YAP1,TAZ和AMPK信号在与高葡萄糖相关的BC进展中的作用.
- 评估向YAP1 / TAZ / AMPK途径在T2D的BC患者中的治疗和预后潜力.
主要方法:
- 追溯分析220公元前患有并发性T2D的患者.
- 免疫组织化学,西部涂抹和qPCR用于评估蛋白质和基因表达 (YAP1,TAZ,AMPK,EMT标记物,自标记物).
- 在体外研究中使用BC细胞系 (T24,UMUC-3) 具有不同的葡萄糖度和体内异种移植小鼠模型.
主要成果:
- 患有BC和T2D的患者表现出较差的病理等级和瘤结节转移阶段.
- 高血糖 (HG) 在体外和体内促进了BC进展,同时抑制了自.
- HG负调节的自标记物和AMPK,与T2D患者的BC样本中的YAP1和TAZ上调相关.
结论:
- 高葡萄糖抑制自并通过YAP1/TAZ/AMPK信号通路促进膀癌的发展.
- 这一途径对于调节BC中葡萄糖失调诱导的自至关重要.
- 针对YAP1/TAZ/AMPK为T2D的BC患者提供治疗潜力和预后价值.
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