相关实验视频

Updated: Jul 17, 2025

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
08:31

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Published on: April 10, 2020

10.1K

全球乙化剖析管道用于快速评估蛋白N-乙转移酶在纤维素中的特异性

Thierry Meinnel1, Jean-Baptiste Boyer2, Carmela Giglione3

  • 1Université Paris Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France. thierry.meinnel@i2bc.paris-saclay.fr.

Methods in molecular biology (Clifton, N.J.)
|September 4, 2023
PubMed
概括

全球乙化分析 (GAP) 使用细菌表达来识别N-乙转移酶 (NAT) 基质. 这种方法通过分析N-终端乙化变化,快速确定NAT基质特异性.

关键词:
乙化 乙化是一种埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.在GNATGNAT中,我们可以看到.不同类的表达式 不同类的表达式这是一种N-乙转移酶.在N-terminomics中,我们可以使用N-terminomics.蛋白质融合是指蛋白质的融合.定量性乙烯基组学研究基质特异性 基质特异性

更多相关视频

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

8.3K
Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

880

相关概念视频

Phase II Reactions: Acetylation Reactions01:24

Phase II Reactions: Acetylation Reactions

Acetylation, a phase II biotransformation reaction, introduces an acetyl group to drugs or their metabolites. Acetyltransferase enzymes facilitate this reaction, which resembles α-amino acid conjugation due to the addition of a functional group to the drug molecule.
The substrates for acetylation are typically drugs or their metabolites with an amino, sulfonamide, or hydrazine functional group. Acetylation can occur at several points in the drug molecule, including primary, secondary, and...
266