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在中国人群中,CYBA C242T多态对孕前病原体的影响

Ru Zhang^1,2, Shuhui Liu³, Ping Tan⁴

¹Department of Medical Genetics, The Affiliated Hospital of Qingdao University, Qingdao, 266000, Shandong, China.

Biochemical genetics

|September 4, 2023

概括

孕前的发病可能涉及与增加CYBA表达相关的氧化应激. 然而,CYBA C242T多态性在研究中华人群中与孕前没有显著的关联.

科学领域:

生物医学研究的研究.
遗传学遗传学是一个
产科产科产科产科产科

背景情况:

孕前 (PE) 的发病因子尚未完全理解,但氧化应激是主要的疑似原因.
p22phox (由CYBA编码) 是NADPH氧化酶的关键组成部分,在氧化应激过程中对于反应性氧物种 (ROS) 生产至关重要.

研究的目的:

为了研究CYBA基因表达,其C242T多态化和孕前之间的关联.
探索CYBA在产前症背后的氧化应激机制中的作用.

主要方法:

分析了PE患者和对照患者的胎盘组织中的CYBA mRNA和蛋白质表达.
在氧化和炎症刺激下 (CoCl2,TNF-α) 检查了HTR-8/SVneo细胞中的CYBA表达.
在大量PE患者和健康对照中使用TaqMan探针对CYBA C242T多态的基因定型.

主要成果:

与对照人群相比,在PE妇女的胎盘中观察到显著增加的CYBA mRNA和蛋白质水平.
在用化和TNF-α刺激的HTR-8/SVneo细胞中,CYBA mRNA表达增加.
在PE病例和对照人群之间,在CYBA C242T基因型或等位基因频率上没有发现显著差异.

结论:

通过氧化应激,CYBA可能会通过氧化应激促进孕前的发病,可能涉及TNF-α等炎症途径.
在研究的中国人群中,CYBA C242T多态性没有与PE相关.
对多样化的种群和额外的CYBA单核酸多态 (SNPs) 进行进一步的研究是有必要的,以充分阐明其作用.

关键词:

CYBA C242T 多形态的多态化基因表达基因表达基因表达在HTR-8中使用.胎盘胎盘胎盘胎盘孕前症孕前症这些细胞是SVneo细胞.

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