尺寸限制是必要的,以确保双分体贝戈莫病毒AC4蛋白的正常运作
Wenwen Zhang1,2, Shunmin Liu1,2,3, Guohui Xie1,2
1Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Molecular plant-microbe interactions : MPMI
|September 4, 2023
概括
较短的AC4蛋白对番茄叶卷病毒 (ToLCHsV) 感染至关重要. 病毒受益于缩短的AC4长度,这有助于叶绿体局部化和病毒传播.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 双胞胎病毒,特别是贝戈莫病毒,编码一个C4/AC4蛋白.
- 虽然通常有80多种氨基酸,但一些病毒AC4蛋白更短,功能意义不明.
- 番茄叶卷曲辛丘病毒 (ToLCHsV) 的AC4蛋白是65个氨基酸,但可以扩展到96.
研究的目的:
- 为了研究ToLCHsV.中的缩写AC4蛋白的功能意义.
- 为了确定AC4长度对病毒感染和蛋白质局部化的影响.
- 了解为什么ToLCHsV保持较短的AC4蛋白质.
主要方法:
- 对ToLCHsV AC4基因进行基因操纵,以创建中断和扩展版本 (EAC4).
- 评估修改后的ToLCHsV的病毒感染性及其对土豆病毒X感染的影响.
- 使用AC4与黄色光蛋白 (AC4-YFP和EAC4-YFP) 融合的亚细胞局部化研究.
主要成果:
- 无论是中断还是延长AC4蛋白都对ToLCHsV感染有害.
- 与野生型AC4相比,扩展的AC4 (EAC4) 显示出减少增强土豆病毒X感染的能力.
- 野生型AC4-YFP局部化在叶绿体上,而EAC4-YFP局部化在细胞外围.
结论:
- 这种AC4蛋白对于ToLCHsV的发病过程至关重要.
- 对病毒感染来说,ToLCHsV AC4的缩短长度可能是有利的,可能是通过促进叶绿体局部化.
- 保持较短的AC4蛋白可能是优化感染效率的病毒策略.
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