从可持续的碳来源最大化微生物生物生产,使用代系统工程
Thomas Eng1, Deepanwita Banerjee1, Javier Menasalvas1
1The Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Cell reports
|September 4, 2023
概括
我们设计了Pseudomonas putida用于生长合的印地戈因的生产,从para-coumarate获得高产. 这种系统生物学方法可以适应各种生物制造应用.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- 为了最大限度地提高异质生物分子的产生,需要了解细胞代谢和调节.
- 增长合策略提高产品标题,产量和生产率.
- 由于代谢模型的局限性,将这些策略应用于非正规碳来源受到阻碍.
研究的目的:
- 开发一种增长合系统,用于在Pseudomonas putida KT2440中使用para-coumarate产生印地基丁.
- 克服与非正规碳流和代谢模型差距相关的挑战.
主要方法:
- 代设计-构建-测试-学习周期超过四次代.
- 探索4,114种潜在的增长合解决方案.
- 实验室进化和集体数据驱动的方法用于细菌菌株的精细化.
- 功能性基因组学和实验验证.
主要成果:
- 工程化Pseudomonas putida KT2440用于从para-coumarate中生长合的印地基因生产.
- 实现了7.3g/L的印地基丁产量,最高理论产量为77%.
- 证明了代设计和数据驱动改进的有效性.
结论:
- 开发的增长合策略非常有效,并且可以在不同的宿主,碳流和产品中推广.
- 这种系统层面的方法为优化生物制造工艺提供了一个强大的框架.
- 这项研究强调了将计算设计与实验验证相结合的力量.
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