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一个比较的全长的转录基因资源提供了对多年生单体群体开花的洞察力

Jiu-Xia Zhao^1,2, Shu Wang^2,3, Jiazhi Liu^2,4

¹CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.

The Plant journal : for cell and molecular biology

|September 4, 2023

概括

研究人员研究了Strobilanthes多年单形群体开花的遗传基础. 他们确定了关键的开花时间基因,为这一关键的生物多样性特征提供了洞察力.

科学领域:

植物生物学植物生物学
基因组学就是基因组学.
进化生物学进化生物学

背景情况:

多年生单形大规模开花是一种重要的进化创新在植物家族,如竹子和Strobilanthes.
驱动这种现象的分子和遗传机制尚未得到充分理解.

研究的目的:

为两个具有对比的开花行为的Strobilanthes物种生成一个全面的转录组资源.
为了确定参与调节多年单形群体开花的分子参与者.

主要方法:

使用Iso-seq和链特异性RNA-seq为Strobilanthes tetrasperma和Strobilanthes biocullata生成的全长转录组数据.
标注非冗余的全长转录和分析的时间空间基因表达模式.
识别了在叶子和树枝上分别表达的转录物,特别是转录因子.

主要成果:

标注分别为S. tetrasperma和S. biocullata的80,971和79,985个全长成绩单.
在S. tetrasperma中鉴定了8794个具有时空表达的转录.
在S. tetrasperma中发现了33种差异表达的转录因子,其中3种 (FAR1相关序列12,核因子Y A1,伪反应调节器5) 可能与开花时间调节途径有关.

结论:

生成的转录组数据为研究大规模开花的遗传基础提供了宝贵的资源.
确定了参与光周期和昼夜时钟通路的候选基因,为了解开花时间多样性提供了线索.
这项研究奠定了进一步研究多年单形群体开花的分子机制的基础.

关键词:

在蓝色的Omics.在ISO-seqqq.在RNA-seqqq.斯特罗比兰提斯 (Strobilanthes) 是一种的植物.多年生单形群体,开花时间长.

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