增加了与混合的Th1/Th2配置和M1/M2配置相关的对脑类动物感染的敏感性,在被循环胺胺免疫抑制的小鼠中
Amanda Miyuki Hidifira1, Anuska Marcelino Alvares-Saraiva1, Elizabeth Christina Perez1
1Programa de Patologia Ambiental e Experimental, Universidade Paulista (UNIP), São Paulo, Brazil.
Experimental parasitology
|September 4, 2023
概括
在C57BL/6小鼠中脑虫虫感染显示,通过改变免疫细胞概况和细胞因子反应,免疫抑制会加剧疾病. 这突显了T和B淋巴细胞在控制微型菌病中的关键作用.
科学领域:
- 免疫学 免疫学 免疫学
- 寄生虫学的寄生虫学
- 微生物学 微生物学
背景情况:
- 脑动物 (Encephalitozoon cuniculi,简称E. cuniculi) 是一种机会主义的微型虫寄生虫,会感染哺乳动物.
- C57BL/6小鼠是研究E. cuniculi的常见模型,但免疫抑制对它们的免疫反应的影响尚未完全理解.
研究的目的:
- 描述感染E. cuniculi的C57BL/6小鼠的免疫反应,有或没有循环胺 (Cy) 诱导的免疫抑制.
- 评估免疫抑制对寄生虫负担和疾病严重性的影响.
主要方法:
- C57BL/6小鼠感染了E. cuniculi,之前是否接受了循环胺的治疗.
- 免疫细胞表型 (巨细胞,T细胞,B细胞) 在脏和腹洗上进行.
- 分析了血清中的细胞因子水平 (Th1,Th2,Th17).
- 对多个器官进行了组织病理学分析,以评估寄生虫负担和组织损伤.
主要成果:
- 单独感染E. cuniculi就导致了M1巨的形状,增加了CD4+,CD8+T细胞,B-1和B-2淋巴细胞,以及Th1/Th17细胞因子的主导地位.
- 经过循环胺治疗的感染小鼠表现出巨细胞数量减少 (M1 概况),T 和 B 淋巴细胞减少,以及混合的 Th1/Th2 细胞因子概况.
- 免疫抑制小鼠表现出更高的真菌负担和更严重的脑类动物感染.
结论:
- 在C57BL/6小鼠中,免疫抑制显著损害了宿主对E. cuniculi的免疫反应.
- 在免疫抑制下,T和B淋巴细胞的减少和细胞因子配置的转变与寄生虫负载增加和疾病严重程度相关.
- 这项研究强调了强大的T细胞和B细胞介导免疫反应对于控制微型菌病的重要性.
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