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识别和验证循环微RNA的参考基因,用于作为妊娠性毒素质症的控制

Ingrid de Siqueira Pereira¹, Allecineia Bispo da Cruz¹, Marta Marques Maia¹

¹Centro de Parasitologia e Micologia, Instituto Adolfo Lutz, Sao Paulo, Brazil; Programa de Pós-Graduação em Ciências da Coordenadoria de Controle de Doenças da Secretaria de Estado da Saúde de São Paulo, Brazil.

Molecular and biochemical parasitology

|September 4, 2023

概括

这项研究确定了miR-484作为一个稳定的内源参考基因,用于量化妊娠性毒素瘤 (GT) 血样本中的微RNA. 这一发现支持准确的生物标志物开发,用于诊断和监测胎儿毒素菌.

科学领域:

生物化学生物化学
分子生物学分子生物学
遗传学遗传学是一种遗传学.

背景情况:

妊娠性毒素菌 (GT) 由于寄生虫的传播,造成了严重的胎儿风险.
循环中的microRNAs (miRNAs) 显示出作为诊断和预后生物标志物的前景.
精确的miRNA量化需要可靠的内源参考基因用于定量实时PCR (qPCR) 的正常化.

研究的目的:

评估三种内源性miRNAs (miR-484,miR-423-3p,miR-26b-5p) 作为正常化剂的稳定性,用于妊娠中毒素瘤的miRNA表达研究.
为了确定最稳定的miRNA,以便在临床样本中准确量化.

主要方法:

分析了来自21名GT妇女和11名健康对照妇女的血样本.
使用RefFinder算法评估miRNA稳定性,包括GeNorm,Normfinder,BestKeeper和比较的三角CT方法.
定量实时PCR (qPCR) 用于miRNA表达分析.

主要成果:

在测试的内源性miRNA中,miR-484表现出最高的稳定性.
miR-484在妊娠中毒素和正常对照组中表现出相当的表达水平.
在多个分析算法中证实了miR-484的稳定性.

结论:

miR-484是一种适合和稳定的内源基因参考基因,用于正常化妊娠中毒素瘤妇女的血样本中的miRNA表达.
这一发现有助于未来研究miRNA生物标志物用于GT诊断和预后.
经过验证的规范化策略对于循环miRNAs可靠的临床应用至关重要.

关键词:

内源的miRNAs是什么规范化规范化规范化定量实时PCR可以使用.毒素菌的发生.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...