瑞佐克托尼亚索拉尼的多种药物耐药性通过增加杀菌剂的排泄量来确定
Xingkai Cheng1, Junting Zhang1, Zhengya Liang1
1Department of Plant Pathology, China Agricultural University, Beijing 100193, China.
Pesticide biochemistry and physiology
|September 4, 2023
概括
在Rhizoctonia solani中,多药性耐药性 (MDR) 是由ATP结合盒 (ABC) 和主要促进体超级家族 (MFS) 运输体驱动的. 这些排水有助于真菌杀菌剂耐药性,影响农业应用.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 植物病原体可能会产生多药耐药性 (MDR),影响疾病管理.
- 在Rhizoctonia solani中MDR的机制,特别是关于流量输送器的机制,需要进一步研究.
- 之前的研究表明,由SYP-14288诱导的R. solani中的MDR涉及ATP结合盒 (ABC) 和主要促进者超级家族 (MFS) 载体.
研究的目的:
- 确认和阐明ABC和MFS载体在Rhizoctonia solani.中MDR发展中的作用.
- 为了识别特定的编码与MDR相关的载体的基因.
- 验证已识别的载体基因在赋予真菌杀菌剂耐药性的作用.
主要方法:
- 转录基因分析 (RNA-Seq),比较野生型和MDR R. solani菌株.
- 在Saccharomyces cerevisiae中,候选载体基因的异质表达.
- 在酵母转化剂中进行真菌杀菌剂敏感性测定和蛋白质产品检测.
主要成果:
- 鉴定了编码六个ABC和七个MFS载体的基因,并在MDR突变中显示出更高的构成性表达.
- 索拉尼基因AG1IA_06082 (ABC) 和AG1IA_08645 (MFS) 的异质表达显著降低了酵母对多种真菌杀菌剂的敏感性.
- 检测到这些基因的蛋白质产物,并且在酵母转化剂中观察到SYP-14288的高积累,证实了它们在MDR中的作用.
结论:
- ABC和MFS载体对Rhizoctonia solani.中多药耐药性的发展有显著的贡献.
- 了解这些载体机制对于管理杀菌剂耐药性和确保有效的疾病控制至关重要.
- 鉴定出来的基因为开发策略提供了目标,以克服植物病原体中的MDR.
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