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死后组织样本的元基因组测序用于识别与新生儿死亡相关的病原体

Vicky L Baillie^1,2, Shabir A Madhi^3,4, Vida Ahyong⁵

¹South Africa Medical Research Council Vaccines and Infectious Diseases Analytics Research Unit, University of the Witwatersrand, Faculty of Health Science, Johannesburg, South Africa. Vicky.Baillie@wits-VIDA.org.

Nature communications

|September 4, 2023

概括

在新生儿死亡中,元基因组测序成功识别了病原体,在90%的病例中证实了传统方法. 这种先进的技术还在81%的不明原因死亡中检测到未知的病原体,揭示了抗菌素耐药性.

科学领域:

医学微生物学医学微生物学
基因组学就是基因组学.
发现病原体发现

背景情况:

新生儿死亡对全球健康构成重大挑战.
对于新生儿感染性死亡的常规诊断方法有时是不够的.
准确识别致病原体对于有效的治疗和预防至关重要.

研究的目的:

评估元基因组下一代测序 (mNGS) 用于识别新生儿死亡的病原体.
将mNGS与传统方法 (培养,核酸放大试验) 进行比较.
探索mNGS在死亡原因不明的病例中的实用性.

主要方法:

在南非索韦托,从新生儿死亡的死后微创组织采样 (血液和肺).
基因组下一代测序的应用.
将mNGS结果与传统的诊断测试进行比较.
确定抗微生物敏感性概况.

主要成果:

在90% (18/20) 的案例中,mNGS结果与传统方法相一致.
在常规方法失败的81% (9/11) 案例中,mNGS发现了假定病原体.
鉴定到的病原体包括Acinetobacter baumannii,Klebsiella pneumoniae,大肠杆菌和Serratia marcescens等.
抗微生物敏感性分析揭示了许多多抗药性生物体.

结论:

甲基因组测序是识别新生儿感染相关死亡病原体的强大工具.
mNGS 增强了超越传统方法的诊断能力,特别是在无法解释的情况下.
该研究强调了mNGS在发现死后样本中抗菌素耐药性模式方面的潜力.

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