关于近红外光蛋白的定量评估
Hanbin Zhang1,2,3, Stavrini Papadaki1,2,3, Xiaoting Sun1,2,3
1School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Nature methods
|September 4, 2023
概括
我们系统地评估了22种近红外光蛋白 (NIR FPs) 用于细胞成像. 确定了表现最好的NIRFP,包括emiRFP670,miRFP680,miRFP713和miRFP720,用于各种成像需求.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 细胞成像 细胞成像
背景情况:
- 近红外光蛋白 (NIRFP) 越来越多地用于生物成像.
- 存在各种各样的NIRFP,这使得研究人员的选择具有挑战性.
- 需要对NIRFP性能进行定量数据,以指导特定应用的选择.
研究的目的:
- 系统地评估和比较22个NIRFP的业绩.
- 为了确定各种细胞和体内成像应用的最佳NIRFP.
- 为选择NIRFP的研究人员提供指导.
主要方法:
- 细胞内亮度,光稳定性,寡合体状态,化学稳定性和细胞毒性的定量评估.
- 在培养的哺乳动物细胞和初级小鼠神经元中进行测试.
- 在模型生物 (C. elegans,斑马鱼,小鼠) 和老鼠肝脏中的体内验证.
主要成果:
- 识别了表现最好的NIRFP:emiRFP670,miRFP680,miRFP713和miRFP720.这些都是最好的NIRFP.
- 这些选定的NIRFP证明了它们适用于大多数成像应用.
- 在小鼠体内成功验证神经元成像和肝脏成像.
- 对多色成像,膨胀显微镜和两光子成像的适用性进行评估.
结论:
- 确定了一组精心策划的高性能NIRFP,用于各种生物成像.
- 该研究提供了关键数据,以帮助研究人员选择最佳的NIR FP.
- 选择的NIRFP是用于先进的成像技术和体内研究的多功能工具.
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