基于SELEX的阿普坦,具有SARS-CoV-2的诊断和进入抑制剂治疗潜力
Sayanti Halder1, Abhishek Thakur2, Supriya Suman Keshry3,4
1Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215, India.
Scientific reports
|September 4, 2023
概括
研究人员开发了一种用于检测和治疗COVID-19的新型aptamer系统. 阿普塔默R有效地结合SARS-CoV-2尖端蛋白,抑制病毒的进入,并显示出显著的抗病毒活性.
科学领域:
- 生物技术是生物技术.
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- COVID-19 流行病的特点是频繁的突变和可变的疫苗疗效.
- 早期检测和制对于控制SARS-CoV-2传播至关重要.
研究的目的:
- 开发一种基于aptamer的系统,具有针对SARS-CoV-2的诊断和治疗潜力.
- 为了识别和表征向SARS-CoV-2尖端蛋白的aptamers.
主要方法:
- 通过指数式丰富对联体的系统演化 (SELEX) 来选适合体库.
- 试验室结合试验和伪型病毒入口试验,以评估阿巴胺结合和特异性.
- 在式对接和分子动力学模拟中分析aptamer-RBD相互作用.
主要成果:
- 鉴定出Aptamer R作为SARS-CoV-2尖端受体结合域 (RBD) 的强有力的结合剂.
- 阿普塔默R证明了SARS-CoV-2伪型病毒入口的特定抑制.
- 亚普坦R和亚普坦J显示显著的抗病毒抑制率分别为95.4%和82.5%.
结论:
- 基于Aptamer的系统显示出对SARS-CoV-2感染的诊断和治疗的前景.
- 阿普塔默R对SARS-CoV-2具有特定的结合性和强大的抗病毒活性.
- 计算分析提供了对阿普坦-RBD相互作用的分子机制的见解.
相关概念视频
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:29Coronavirus
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