快速生长的Vibrio natriegens的代谢工程,以实现高效的pyruvate生产
Fengli Wu1,2, Shucai Wang3,4,5, Yanfeng Peng3,4
1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. wu_fl@tib.cas.cn.
Microbial cell factories
|September 4, 2023
概括
经过工程设计的Vibrio natriegens产生高滴度的pyruvate,这是一个关键的代谢中心化学物质. 这种快速生长的细菌实现了高生产率,为有效的酸盐及其衍生物生物合成铺平了道路.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- 酸盐是一种重要的附加值化学和代谢枢纽.
- 一种快速生长的细菌Vibrio natriegens由于高基质吸收,显示出合成生物学的潜力.
- 研究V. natriegens用于高生产率的酸盐生产.
研究的目的:
- 为了改造一种V. natriegens菌株以提高酸盐的产量.
- 为了利用V. natriegens在工业应用中的高基板吸收率.
主要方法:
- 删除prophage基因集群和副产品生物合成基因.
- 对aceE (pyruvate dehydrogenase E1组成部分) 的下调,以重定向碳流.
- 为平衡生长和酸盐合成,精细调整ppc (酸酸碳酸酶) 表达式.
主要成果:
- 工程菌株PYR32在16小时内从葡萄糖中产生了54.22g/L的pyruvate.
- 达到1.17mol/mol的酸盐产量和3.39g/L/h的生产率.
- 证明了糖和葡萄糖酸盐的有效转化为酸盐.
结论:
- 一种新的V. natriegens菌株成功地被用于高生产率的酸盐合成.
- 这项工作确立了V. natriegens作为一种可行的底盘,用于pyruvate和衍生生物合成.
- 奠定了未来微生物生产基于酸盐的化学品的基础.
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