皮竞争的内源RNAs调节主要农业害虫的杀虫剂透和耐药性
Li-Wei Meng1,2, Guo-Rui Yuan1,2, Meng-Ling Chen1
1Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400716, China.
BMC biology
|September 4, 2023
概括
抗杀虫剂耐药性是农业的主要威胁. 一项新的研究揭示,昆虫的皮质是昆虫的皮质.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 农药耐药性对农业和人类健康构成重大威胁.
- 了解抗杀虫剂耐药性机制对于有效的害虫管理至关重要.
- 昆虫皮质在延迟异生菌透中的作用是最近的发现,但机制仍然不清楚.
研究的目的:
- 研究昆虫对抗虫剂耐药性的贡献.
- 阐明了皮质介导的昆虫杀虫剂透耐药性背后的分子机制.
- 确定涉及皮层厚度和抗昆虫剂耐药性的调节途径.
主要方法:
- 研究了东方果菌株的皮层厚度和杀虫剂透率之间的关系.
- 使用的microRNA (miR-994) 模仿和抑制治疗,以评估其对皮肤和敏感性的影响.
- 分析了长非编码RNA (lnc19419) 在调节miR-994/CPCFC通路中的作用.
主要成果:
- 覆盖物占杀虫剂耐药性的40%以上,更厚的皮质减少了透.
- miR-994通过向皮膜蛋白 (CPCFC) 直接影响皮膜厚度和杀虫剂易感性.
- lnc19419在miR-994的上游作用,间接上调CPCFC并促进耐药性.
结论:
- 一个调节轴 (lnc19419~miR-994~CPCFC) 控制皮层厚度和抗虫剂透性.
- 竞争的内源RNA网络调节昆虫皮层厚度,从而赋予昆虫杀虫剂耐药性.
- 这些发现提供了关于昆虫抵抗机制和潜在的害虫控制策略的见解.
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