在家用子中对致病性Candida spp. 的遗传学分析
Forough Talazadeh1, Masoud Ghorbanpoor2, Milad Masoudinezhad1
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Veterinary research forum : an international quarterly journal
|September 5, 2023
概括
这项研究调查了子白病,发现19%的疑似鸟类感染了Candida物种. 遗传学分析确定了Candida albicans和Candida glabrata,突出了受影响的子中常见的临床症状.
科学领域:
- 兽医真菌学 兽医真菌学
- 鸟类病理学 鸟类病理学
- 分子诊断学 分子诊断学
背景情况:
- 子白病,是由 *Candida spp. 引起的 *,在鸟类种群中是一个重要的健康问题.
- 临床症状包括抗生素耐药性腹,作物停滞和口腔病变,需要准确的诊断.
- *Candida spp. 的遗传学特征 *在子中,对于理解菌株多样性和流行病学至关重要.
研究的目的:
- 为了确定疑似感染的子中角膜炎的流行率.
- 为了隔离和识别 *Candida spp. *使用微生物学和分子方法.
- 对已识别的 *Candida spp. 进行遗传学分析. * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
主要方法:
- 在伊朗阿瓦兹,从100只疑似子中收集了口和口的抽样.
- 对 *Candida spp. 的隔离和鉴定 *使用Sabouraud乳糖和显微镜检查.
- 使用聚合酶链反应 (PCR) 和DNA测序进行确认和基因表征.
主要成果:
- 显微镜和培养方法表明19%的子患有白病.
- 聚合酶链反应 (PCR) 证实 *Candida spp. * 在19%的采样鸟类中.
- 遗传学分析确定这些分离物为*Candida albicans*和*Candida glabrata*,其序列与全球菌株具有很高的相似性.
结论:
- 在研究的人群中,子候群病很普遍, *Candida albicans* 和 *Candida glabrata* 是病原体.
- PCR提供了一种可靠的方法来确认 *Candida spp. * 子感染的情况.
- 鉴定到的 *Candida* 菌株与来自各个国际地区的分离品具有显著的遗传相似性,这表明有可能在全球传播.
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