高流动性组第1框 (HMGB1) 在小鼠尾管中的发育表达
Wenjing Liu1, Shanshan Ming2, Xiaobing Zhao2
1Department of Otorhinolaryngology-Head and Neck Surgery, Zhongda Hospital, Southeast University, Nanjing. liuwj38@163.com.
European journal of histochemistry : EJH
|September 5, 2023
概括
高流动性组盒1 (HMGB1) 在正在发育的小鼠耳中表达,特别是在Corti.的器官中. 它与增殖细胞的同定位表明HMGB1在耳发育中的作用.
科学领域:
- 听觉神经科学 听觉神经科学
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 高流动性群体盒1 (HMGB1) 与噪音引起的听力损失有关.
- 在开发过程中,HMGB1在听觉系统中的特定功能尚不清楚.
研究的目的:
- 研究HMGB1在小鼠耳发育过程中的表达模式和潜在作用.
- 检查HMGB1定位从胚胎18.5天到产后28天.
主要方法:
- 在冷的老鼠耳截面上进行双重免疫光.
- 与发育阶段和特定细胞类型相关的HMGB1表达的分析.
- 与基-67增殖标记和细胞特异标记 (Sox2,TUJ1,Sox10) 进行同局部化研究.
主要成果:
- HMGB1在耳细胞的核中得到广泛表达,包括Corti的器官.
- HMGB1表达显示出发育调节,存在于耳边缘细胞,大上皮脊 (GER) 和支细胞中.
- 在胚胎和出生后早期发育期间,HMGB1与各种耳区域的增殖细胞 (Ki-67) 共定位,包括新生儿质细胞.
结论:
- 在小鼠耳发育过程中,HMGB1表现出明显的时空表达模式.
- HMGB1与增殖细胞的关联表明它参与了耳发育过程.
- 需要进一步的研究来阐明HMGB1在听觉系统发展中的精确机制.
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