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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.4K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

5.5K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.5K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K

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CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

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Cryo-EM: the revolution continues.

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Measurement of atomic scattering factors by cryoelectron microscopy.

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CryoEM Structures of Native Quinol-Dependent Nitric Oxide Reductase in Resting and Quinol-Bound States.

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Accurate atomic resolution XFEL structures of a metalloenzyme reveal key insights into its catalytic mechanism.

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相关实验视频

Updated: Jul 17, 2025

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

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"冷电磁显微镜":电子冷显微镜,冷电子显微镜或其他什么?

Richard Henderson1, Samar Hasnain2

  • 1MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.

IUCrJ
|September 5, 2023
PubMed
概括

低温电子显微镜 (cryoEM) 提供了对蛋白质结构和机制的强大洞察力. 这项研究要求对冷EM制定统一的定义,以标准化科学文献.

科学领域:

  • 结构生物学是结构生物学.
  • 生物化学 生物化学
  • 生物物理学的生物物理.

背景情况:

  • 结构生物学工具的进步为复杂的生物系统提供了前所未有的洞察力.
  • 电子显微镜 (cryoEM) 显著改善了对膜蛋白和大型复合体的高分辨率结构的确定.
  • 高分辨率的冷EM数据 (约2 Å) 现在允许对酶机制进行详细的研究.

研究的目的:

  • 为了解决围绕缩写cryoEM的定义的模两可.
  • 鼓励社区参与澄清"冷EM"一词.
  • 为了统一使用cryoEM的日益增长的科学文献.

主要方法:

  • 关于冷EM术语的当前文献和实践的审查.
  • 社区协商和讨论以建立共识定义.
  • 分析术语模两可对科学传播的影响.

主要成果:

  • 缩写cryoEM随着时间的推移被多种方式解释.
  • 缺乏标准化的定义阻碍了对冷EM研究的一致解释.
  • 统一的定义对于不断扩大的冷EM文献的清晰性和可访问性至关重要.
关键词:
化电子显微镜技术的电子显微镜冰冷化EMEM可以使用.电子冷显微镜的使用编号表中的名称.标准化 标准化 标准化

更多相关视频

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

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Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
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Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Published on: November 20, 2021

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相关实验视频

Last Updated: Jul 17, 2025

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
09:16

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Published on: February 7, 2022

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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

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Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
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Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Published on: November 20, 2021

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结论:

  • 需要一个明确和普遍接受的冷EM的定义.
  • 社区的共识将加强结构生物学研究的标准化.
  • 标准化冷EM术语将提高科学发现的可发现性和影响.