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通过扰乱血管发育 (在植物中) 来诱导瘤症状的新型病毒因子的识别

Go Atsumi^1,2,3, Satoshi Naramoto⁴, Masahiro Nishihara¹

¹Iwate Biotechnology Research Center , Kitakami, Iwate, Japan.

Journal of virology

|September 5, 2023

概括

一种科布-肖相关病毒基因片段,科布-肖诱导因子 (KOBU),在植物中引起胆汁形成和宫外血管和叶子发育. 这一发现提供了关于植物病毒相互作用和发育途径的见解.

科学领域:

植物病理学植物病理学
分子植物病毒学分子植物病毒学
植物生长生物学植物生长生物学

背景情况:

植物病毒对农业造成重大损失,但疾病机制往往不清楚.
科布-肖病在珍中涉及胆汁形成和子宫外血管组织的发展.

研究的目的:

为了研究基因科布肖相关病毒诱导疾病症状的分子机制.
为了确定负责胆汁和子宫外组织形成的特定病毒因素.

主要方法:

病毒基因片段 (KOBU) 在模型植物 (Nicotiana benthamiana,Arabidopsis thaliana和) 中的表达.
转基因植物的表型分析,包括胆汁形成和宫外组织分化.
转录组分析以确定受影响的信号通路.
对KOBU蛋白的亚细胞局部化研究.

主要成果:

科布基因片段诱导了N. benthamiana的胆汁形成和宫外膜发育.
转基因Gentian表达KOBU显示出瘤症状.
KOBU表达也导致了额外的叶状组织的分化.
转录组数据表明,KOBU激活了体发育途径.
KOBU蛋白定位在核中,并与mRNA拼接因子共同定位.

结论:

科布是一种新型的,从属科布-肖相关病毒的类毒性因子.
科布刺激血管和叶子的发育,为植物病毒相互作用提供了新的见解.
了解KOBU的机制可以阐明植物血管和叶子发育调节.

关键词:

珍提亚三植物 × G. 斯卡布拉植物病毒植物病毒血管发育血管发育毒性因子是毒性因子.

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