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在斑马鱼中使用单链DNA和基因编辑工具的突变敲入方法

Sergey V Prykhozhij1, Jason N Berman2,3

  • 1Children's Hospital of Eastern Ontario Research Institute, Ottawa, ON, Canada.

Methods in molecular biology (Clifton, N.J.)
|September 5, 2023
PubMed
概括

斑马鱼的CRISPR/Cas9基因编辑使用单链DNA (ssDNA) 模板进行精确的基因组修改. 这篇评论详细介绍了ssDNA方法,挑战和解决方案,用于生成突变斑马鱼系.

关键词:
这就是CRISPR/Cas9的作用.疾病模型的疾病模型.敲进来 - 敲进来长时间的ssDNADNA变异点的变异点是指变异点的变化点.斑马鱼是一种斑马鱼.这就是SSODNN.

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