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相关概念视频

Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
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在斑马鱼中使用MIC-Drop进行可扩展的CRISPR屏幕.

Saba Parvez1, Tejia Zhang1, Randall T Peterson2

  • 1Department of Pharmacology & Toxicology, University of Utah, Salt Lake City, UT, USA.

Methods in molecular biology (Clifton, N.J.)
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概括

多重复合混合CRISPR滴 (MIC-Drop) 能够在斑马鱼体内进行大规模的体内遗传查. 这种新的平台克服了以前的挑战,使基因功能研究更容易获得和更有效.

关键词:
克里斯普尔-Cas9是什么意思F0 在CRISPR屏幕上遗传屏幕是一种遗传屏幕.高通量的高通量.在生物体内,在生物体内.反向遗传学是一种反向的遗传学.斑马鱼是一种斑马鱼.

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科学领域:

  • 遗传学 遗传学是一种遗传学.
  • 分子生物学分子生物学
  • 斑马鱼模型 斑马鱼模型

背景情况:

  • 克里斯普尔-Cas9是基因功能研究的关键技术.
  • 由于成本和劳动力,大型体内遗传查很困难.
  • 之前的方法限制了对整个生物体的系统基因查询.

研究的目的:

  • 为大规模的斑马鱼逆转基因选提供一个新的平台.
  • 详细介绍一个实施多重复合混合CRISPR滴滴 (MIC-Drop) 的协议.
  • 为了促进高效的 in vivo 基因功能分析.

主要方法:

  • 开发一个MIC-Drop平台.
  • 使用CRISPR-Cas9进行多重遗传干扰.
  • 在斑马鱼中进行大规模查的应用.

主要成果:

  • MIC-Drop使可扩展的体内基因查成为可能.
  • 该平台简化了生成和跟踪突变动物的过程.
  • 促进对整个生物体中的基因功能进行系统的询问.

结论:

  • MIC-Drop显著提高了斑马鱼大规模逆转基因选的可行性.
  • 这项技术减少了 in vivo 遗传研究的障碍.
  • 能够更全面地了解复杂生物系统中的基因功能.