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Updated: Jul 17, 2025

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

使用表面等离子体共振研究SH2域-相互作用.

Gabrielle M Watson¹, Menachem J Gunzburg², Jacqueline A Wilce³

¹The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.

Methods in molecular biology (Clifton, N.J.)

|September 5, 2023

概括

表面等离子体共振生物传感器精确测量蛋白相互作用,提供动力和亲和数据,无需样本标记. 本研究详细介绍了使用这种敏感技术来分析对Grb7-SH2域的结合.

关键词:

GST融合蛋白的表达方式结合生长受体的蛋白质 7 增长受体结合蛋白传感器芯片的准备工作稳定状态分析稳定状态分析表面等离子体共振是什么?

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相关实验视频

Last Updated: Jul 17, 2025

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

科学领域:

生物化学生物化学
分子生物学分子生物学
生物物理学的生物物理.

背景情况:

-蛋白相互作用在细胞信号传递中至关重要.
准确的动力和亲和数据对于理解这些相互作用至关重要.
无标签的生物传感技术在样本保存和分析方面具有优势.

研究的目的:

应用表面等离子体共振 (SPR) 生物感知来精确评估与Grb7-SH2域的相互作用.
为了证明SPR可靠性,实时比较各种和SH2域.
提供使用BIAcore系统收集和分析数据的详细方法.

主要方法:

利用了表面等离子体共振 (SPR) 生物传感与BIAcore系统.
准备 Grb7-SH2 域与 GST 标签在传感器芯片上固定.
在不同的溶液条件下对结动力学和亲和力进行实时分析.

主要成果:

成功且可靠地测量了与Grb7-SH2域的相互作用.
能够直接比较多个的结合特性.
促进了对不同SH2域的的实时比较.

结论:

SPR生物感知是一种强大而敏感的方法,用于表征蛋白相互作用.
描述的方法提供了一个强大的平台,用于分子结合的定量分析.
这些原则可转移到其他生物传感器平台,用于各种生物应用.