相关实验视频

Updated: Jul 17, 2025

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Published on: February 18, 2017

用流细胞计和细胞分类来分离人类BCAM阳性角膜原生细胞的协议

Yuzuru Sasamoto¹, Philip C Yeung², Johnathan Tran³

¹Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA; Transplant Research Program, Boston Children's Hospital, Boston, MA, USA.

|September 5, 2023

概括

这项研究详细介绍了一项隔离BCAM阳性基底四肢上皮细胞的协议,这对角膜再生至关重要. 这些细胞使用流细胞计来识别,以更好地了解角膜上皮分化.

科学领域:

眼科医生眼科眼科
细胞生物学细胞生物学
再生医学是一种再生医学.

背景情况:

基底四肢上皮细胞对于角膜表面的维护和再生至关重要.
具有BCAM阳性的细胞代表了具有全克隆形成能力的早期过渡放大细胞群 (TAC).
了解这些细胞是开发角膜修复策略的关键.

研究的目的:

提出一个详细的协议,以隔离BCAM阳性细胞从人类捐赠者的角膜.
为了进一步研究调节角膜再生的因素.
为了促进角膜上皮分化和全克隆形成的研究.

主要方法:

人体捐赠角膜被切割和分离.
细胞被染色为流细胞计的特定抗体.
用细胞分类来净化BCAM阳性和BCAM阴性细胞.
纯化的细胞被培养为全克隆和细胞叶形成试验.

主要成果:

建立了一个可靠的协议,以隔离BCAM阳性基底四肢上皮细胞.
该协议允许分离BCAM阳性和BCAM阴性细胞群.
隔离的细胞适合用于与角膜再生相关的功能测试.

结论:

本方案有效隔离BCAM阳性细胞,这是角膜再生的关键群体.
这种方法支持研究支角膜上皮分化的细胞机制.
这些发现有助于推进眼科和再生医学方面的研究.

关键词:

细胞生物学细胞生物学细胞隔离细胞隔离基于细胞的测试流量细胞计/质量细胞计.干细胞是干细胞.

更多相关视频

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Published on: April 16, 2013

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

Published on: August 21, 2014

相关概念视频

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.