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在生物克隆的子菌株中,有证据表明先前存在的子子菌株多样性
Tess Gunnels1, Ronald A Shikiya1, Taylor C York1
1Department of Medical Microbiology and Immunology, Creighton University, Omaha, Nebraska, United States of America.
PLoS pathogens
|September 5, 2023
概括
子疾病,致命的神经退行性疾病,涉及子菌株. 这项研究表明,主导的子菌株抑制子菌株,当主导菌株减少时,可以出现子菌株.
科学领域:
- 神经退行性疾病的神经退行性疾病
- 子生物学 子生物学
- 分子生物学分子生物学
背景情况:
- 病是致命的神经退行性疾病,影响哺乳动物,由PrPSc引起.
- 子菌株是由遗传性疾病表型来定义的.
- 性疾病中的耐药性表明存在主导菌株和子菌株.
研究的目的:
- 为了调查子菌株包括主导菌株和子菌株的假设.
- 探索子子株出现的条件.
- 了解子进化和药物耐药性的机制性影响.
主要方法:
- 生物化学方法可选择性地减少感染传染性小鹿脑病变 (TME) 的大脑的昏昏欲睡 (DY) PrPSc.
- 与DY TME相比,分析新兴菌株的改变性质.
- 连续通过实验来评估子的稳定性.
主要成果:
- 通过对主导DY PrPSc进行选择性降低,可以产生具有独特性质的亚株.
- 在DY TME感染的大脑中已经存在的新兴亚菌株,在选择过程中没有形成.
- DY TME表现出生物稳定性,即使在高位序列传递下也是如此.
结论:
- 主导的子菌株抑制子菌株,这些子菌株可以在主导群体内存在.
- 亚菌株可能是子的共同特征,当主导菌株抑制得到缓解时就存在.
- 这些发现对了解子菌株演变,耐药性和物种间传播有重要意义.
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