通过TET3介导的DNA氧化对于肠道上皮细胞对压力因素的反应至关重要
Edward A Gonzalez1, Yue Liu1, Dahui Wang1
1Department of Biological Sciences, Rutgers University-Newark, Newark, NJ 07102.
概括
十-十一转位3 (TET3) 通过调节肠道细胞中的DNA甲基化和基因表达来维持肠道健康至关重要. TET3 保护病原体和化学压力因素,帮助上皮质修复和平衡.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 胃肠病学 胃肠病学
背景情况:
- 基因甲基化是一种关键的表观遗传调节剂.
- 十-十一转位 (TET) 酶通过氧化5-甲基细胞素来逆转DNA甲基化.
- TET酶在肠道平衡和对压力因素的反应中的作用在很大程度上是未知的.
研究的目的:
- 研究TET3在小鼠肠道上皮分化的作用.
- 确定TET3对肠道对光线压力因素的反应的贡献.
- 阐明TET3对肠道平衡的影响.
主要方法:
- 产生的小鼠与肠上皮细胞特异性切除的Tet3.3.
- 分析了野生类型和Tet3缺乏的小鼠的转录组.
- 评估了对肠道病原体感染和硫酸 (DSS) 诱导的大肠炎的敏感性.
- 进行了全基因组的5-基甲基氨酸 (5hmC) 分析.
主要成果:
- Tet3 除损害了帕内斯细胞分化,上皮再生和先天免疫反应.
- 缺乏Tet3的小鼠对感染和DSS结肠炎的敏感性增加.
- 感染增加了5hmC的丰度,而DSS则减少了它.
- 5hmC丰富转向了Notch,Wnt和自途径中的基因.
结论:
- TET3对于肠道上皮的分化和再生至关重要.
- TET3在保护肠道免受病原体和化学压力因素的影响方面发挥着至关重要的作用.
- TET3调节肠道上皮细胞DNA甲基组和转录组,维持组织平衡.
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