氨酸乳化调节了Streptococcus mutans的代谢途径和生物膜的形成
Zhengyi Li1, Tao Gong1, Qinrui Wu1,2
1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Science signaling
|September 5, 2023
概括
蛋白质的乳酸修饰 (lysine乳酸化) 调节了细菌的新陈代谢. 在Streptococcus mutans中,这一过程通过修改RNA聚合酶来影响生物膜的形成,为 prokaryotic 代谢调节提供了新的见解.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 氨酸乳酸化 (Kla) 是一种后翻译性修饰,调节了真核生物中的代谢过程.
- 克拉在 prokaryotic 代谢调节中的作用仍然在很大程度上未被探索.
研究的目的:
- 为了研究氨酸乳酸化在 cariogenic 细菌Streptococcus mutans中的分布和功能.
- 探索糖的可用性,代谢状态和S. mutans中的Kla之间的联系.
主要方法:
- 在低糖和高糖条件下对S. mutans中的乳酸蛋白和部位进行比较分析.
- 在体外酶分析以确定GNAT13.的乳酸转移酶活性.
- 在GNAT13过度表达或RpoA修改后对生物膜形成的评估.
主要成果:
- 在469种蛋白质中确定了1869个氨酸乳化位点,在促进生物膜的条件中更高的流行率.
- 高糖增加了全球的Kla,但降低了RpoA Lys173的乳糖化,抑制了细胞外多糖合成.
- GNAT13显示了氨酸乳酸转移酶活性,特别是乳酸RpoA Lys173,抑制了生物膜的形成.
结论:
- 氨酸乳酸化在S. mutans中很普遍,受糖的可用性和生长状态的影响.
- 在Lys173中由GNAT13对RpoA的特异性乳化是抑制生物膜形成的关键调节机制.
- 这项研究阐明了乳酸在 prokaryotic 代谢调节和 S. mutans 毒性的作用.
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