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相关概念视频

Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
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Protein Modifications in the RER

Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
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Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...

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相关实验视频

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Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
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使用ectodomain抗体操纵PTPRD功能

Zhe Qian1,2, Dongyan Song1, Jonathan J Ipsaro3

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.

Genes & development
|September 5, 2023
PubMed
概括
此摘要是机器生成的。

针对受体蛋白氨酸酸酶三角体 (RPTPδ/PTPRD) 的抗体抑制其活性并促进降解,抑制促进瘤信号和细胞入侵.

关键词:
这是PTPRD.在SRC中,它是SRC.这是一种双价抗体.分化二元化是指二元化的过程.蛋白质氨酸酸化蛋白质的氨酸酸化.接收蛋白质氨酸酸酶的受体.

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科学领域:

  • 分子生物学分子生物学
  • 癌症研究 癌症研究
  • 药物开发 药物开发

背景情况:

  • 蛋白氨酸酸酶 (PTPs) 调节信号转导,但它们是未被充分研究的药物标.
  • 受体蛋白氨酸酸酶三角酶 (RPTPδ/PTPRD) 通过增加SRC活性,促进瘤进展和转移.
  • 受体蛋白氨酸酸酶 (RPTPs) 的抑制与二分化有关.

研究的目的:

  • 开发针对RPTPδ/PTPRD细胞外域的抗体,以调节二分化和信号传递.
  • 研究向癌症中的RPTPδ/PTPRD的治疗潜力.

主要方法:

  • 对PTPRD外域产生抗体.
  • 在转移性乳腺癌细胞中对内源性PTPRD的抗体结合的验证 (CAL51).
  • 评估抗体诱导的酸酶活性,二分化和降解途径 (溶酶和蛋白质酶) 的变化.

主要成果:

  • 单克隆抗体RD-43结合了内源性PTPRD,抑制了其酸酶活性,并诱导了PTPRD降解.
  • RD-43触发了PTPRD的二元化,从而损害了催化活性.
  • 抗体介导的PTPRD降解通过 lysosomal 和 proteasomal 途径发生,独立于分泌酶裂解.
  • RD-43治疗抑制了SRC信号传递和PTPRD依赖的细胞入侵.

结论:

  • 用抗体准细胞外RPTPδ/PTPRD可以抑制其促进瘤的功能.
  • 抗体诱导的二分化和随后的RPTPδ/PTPRD降解是可行的治疗策略.
  • 这种方法对RPTPδ/PTPRD驱动的癌症具有治疗潜力.