通过CO2扩散到人类精子中控制细胞内pH和二碳酸盐
Elena Grahn1, Svenja V Kaufmann2, Malika Askarova1
1Max Planck Institute for Neurobiology of Behavior-caesar, Molecular Sensory Systems, Ludwig-Erhard-Allee 2, 53175, Bonn, Germany.
Nature communications
|September 5, 2023
概括
人类精子中的二碳酸盐生产,对于运动和受精至关重要,是由CO2扩散和细胞内pH值变化调节的,而不是直接的输送器. 这一发现为男性不孕症提供了新的见解.
科学领域:
- 生殖生物学 生殖生物学
- 精子生理学 精子生理学
- 细胞信号传输 细胞信号传输
背景情况:
- 从CO2和H2O中形成二碳酸盐 (HCO3-) 和H+对于精子运动和受精至关重要,由cAMP合成介导.
- 一个复杂的信号网络控制着这种反应,细胞内pH (pHi) 和HCO3调节的关键参与者尚未在人类精子中完全确定.
研究的目的:
- 在人类精子中识别调节细胞内pH (pHi) 和碳酸盐 (HCO3-) 的关键蛋白质.
- 阐明HCO3-生产的机制及其对精子功能的影响.
主要方法:
- 量化质谱法 (MS) 用于识别参与pHi和HCO3-调节的蛋白质.
- 动力补丁式光测量被用来研究传送器功能和细胞反应.
主要成果:
- 人类精子中的休息pHi主要通过对氨酸敏感的Na+/H+交换来调节.
- 精子特定的Na+/H+交换器SLC9C1不受电压或cAMP的限制.
- 双碳酸盐通过二氧化碳扩散和随后的平衡转移产生,没有发现任何显著的HCO3-载体.
- 质子通道Hv1可以充当单向门,以减轻来自HCO3合成的酸化.
结论:
- 人类精子的HCO3-调节依赖于CO2扩散和细胞内pH动态,而不是专门的HCO3-载体.
- 鉴定的机制为了解精子功能和男性不孕症提供了一个新的框架.
- 这项研究强调了SLC9C1和HV1在精子生理学中的作用.
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