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Updated: Jul 17, 2025

Murine Dermal Fibroblast Isolation by FACS

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

功能上不同的与癌症相关的纤维细胞亚群在状细胞癌中建立了一个促进瘤的环境

Sabrina Schütz¹, Llorenç Solé-Boldo¹, Carlota Lucena-Porcel^2,3

¹Division of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Center, 69120, Heidelberg, Germany.

Nature communications

|September 5, 2023

概括

与癌症相关的纤维细胞 (CAF) 促进皮肤状细胞癌 (cSCC) 的生长. 这项研究确定了来自特定纤维细胞群的独特CAF亚型,揭示了它们在cSCC瘤微环境中的作用.

科学领域:

在瘤学瘤学.
皮肤病学皮肤病学
细胞生物学细胞生物学

背景情况:

皮肤状细胞癌 (cSCC) 是一种具有转移潜力的流行性皮肤癌.
cSCC的进展涉及皮肤纤维细胞激活为癌症相关纤维细胞 (CAF),但其起源和功能尚不清楚.

研究的目的:

研究皮肤状细胞癌 (cSCC) 中癌症相关纤维细胞 (CAF) 的起源和功能亚型.
了解CAFs在建立特定于cSCC的亲瘤微环境中的作用.

主要方法:

分析了来自健康皮肤的115,000多个单细胞转录组,波恩病 (BD) 和来自男性捐献者的cSCC样本.
对CAF亚型及其在瘤微环境中的相互作用进行比较分析.

主要成果:

在cSCC中识别出不同的免疫调节和矩阵重塑CAF亚型.
这些CAF亚型分别来源于亲炎性纤维细胞和介酶体纤维细胞.
CAF亚型在行动性角质炎 (AK) 中基本上不存在,并且在cSCC特定的瘤微环境中发挥着关键作用,与基底细胞癌不同.

结论:

该研究阐明了cSCC中的皮肤CAF的潜在起源和功能多样性.
这些发现为针对cSCC微环境的向治疗提供了关键的见解.

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