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Circ_0005736通过miR-636/MAPK1轴促进肌衍生干细胞的基分化

Guangzhao Yang¹, Fei Chen², Chunyan Zhang²

¹Department of Sports, Communication University of China, Nanjing, No.26, Pengshan Road, Jiangning District, Nanjing, 211172, China. yanggz@cucn.edu.cn.

Journal of orthopaedic surgery and research

|September 5, 2023

概括

循环RNAcirc_0005736通过调节miR-636/MAPK1轴,促进肌干细胞的基分化. 这一发现为旋转手套肌病变提供了潜在的治疗点.

科学领域:

生物化学生化学
细胞生物学细胞生物学
再生医学是一种再生医学.

背景情况:

肌衍生干细胞 (TDSCs) 具有强大的克隆原性和原性能力.
在旋转手套肌病症中观察到降低的circ_0005736水平.
需要阐明circ_0005736在TDSC阴性分化中的作用.

研究的目的:

为了研究circ_0005736在TDSC中基分化的功能.
探索circ_0005736在肌愈合中的作用背后的分子机制.

主要方法:

使用转化生长因子β1 (TGF-β1) 诱导TDSC进行基分化.
用CCK-8,EDU,transwell和伤口愈合试验来评估细胞增殖,入侵和迁移.
基因和蛋白质表达水平通过qRT-PCR和西班牙血栓量化;使用双露西法酶记者和RIP测定证实了分子相互作用.

主要成果:

治疗TGF-β1增强了TDSC的基分化,扩散,入侵和迁移.
在TGF-β1治疗时,Circ_0005736的表达增加了;它的敲击抑制了基分化.
大约0005736个海绵miR-636,对MAPK1表达进行上调,是miR-636.6的标. 救援测试证实了circ_0005736/miR-636/MAPK1轴的作用.

结论:

Circ_0005736促进TGF-β1诱导的TDSCs中的基分化.
这种促进通过增强肌特定标记物的产生,扩散,入侵和迁移而发生.
这种机制涉及circ_0005736/miR-636/MAPK1轴.

关键词:

在MAPK1中,MAPK1是MAPK1.身体活动身体活动 TDSC TDSC是什么意思代性差异化分化方式环_000573636 周围的时间在 miR-63636 中使用.

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