抑制天体细胞连xin-43 半通道阻断辐射诱导的囊泡VEGF-A释放和血脑屏障功能障碍

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Steffi Schumacher¹, Hanane Tahiri¹, Pascal Ezan²

¹Department of Basic and Applied Medical Sciences - Physiology Group, Ghent University, Ghent, Belgium.

|September 6, 2023

概括

大脑辐射会通过天体细胞VEGF-A释放导致屏障泄漏. 准连线43 (Cx43) 半通道可以防止这种情况,为辐射副作用提供治疗策略.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
辐射瘤学辐射瘤学

背景情况:

治疗性脑辐射可以引起副作用,如血脑屏障泄漏.
星球细胞通过从它们的末端足部释放血管活性物质来促进这种泄漏.

研究的目的:

为了研究X射线辐射对天体细胞囊泡运输的影响.
为了确定操纵星细胞连接体是否会影响辐射诱导的屏障泄漏.

主要方法:

在小鼠中研究了X射线对天体细胞囊泡运输的影响.
使用了连接素43 (Cx43) 淘汰和突变小鼠.
使用TAT-Gap19.19研究了Cx43半通道开放的作用.

主要成果:

辐射导致VEGF-A载荷的VAMP3囊泡重组并向脚端移动.
从天体细胞末脚释放的VEGF-A导致屏障泄漏,取决于和Cx43.
在Cx43淘汰,Cx43突变小鼠和Cx43半通道抑制中,可以防止囊泡运输,VEGF-A释放和泄漏.

结论:

天体细胞VEGF-A释放是脑辐射并发症的关键因素.
抗VEGF治疗可以减轻这些并发症.
准Cx43半通道可以防止天体细胞在辐射后释放VEGF.

关键词:

星球细胞是星球细胞.血液障碍物 - 血液障碍物大脑大脑大脑的大脑大脑和是最重要的.连接连接连接电离辐射辐射的电离辐射.

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