在南瓜中进行全基因组调查和微卫星的表征,并设计基于网络的微卫星数据库:CMDBDB
Siddanna Savadi1, B M Muralidhara2, V Venkataravanappa2
1ICAR- Directorate of Cashew Research (DCR), Puttur, Karnataka, India.
Frontiers in plant science
|September 6, 2023
概括
这项研究提出了第一个全面的基因组范围的微卫星 (SSRs) 在果的表征. 它开发了多态SSR标记物和一个用户友好的数据库,用于果仁遗传研究和育种.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 植物科学 植物科学
背景情况:
- 果仁 (Anacardium occidentale) 是一种具有经济意义的重要作物,具有多样化的应用.
- 目前在果仁中缺乏全基因组微卫星 (简单序列重复 - SSR) 特性.
- 在植物的遗传研究和育种中,SSR标志物至关重要.
研究的目的:
- 执行第一个全面的基因组范围的SSRs在南瓜基因组的表征.
- 开发多态SSR标记物用于果仁遗传分析.
- 为SSR可访问性建立一个基于Web的数据库 (Cashew微卫星数据库 - CMDB).
主要方法:
- 用于全基因组SSR发现和表征的生物信息分析.
- 在矿开采的SSR中进行PCR查,用于在南瓜基因型中验证多态性.
- 开发一个基于Web的可搜索数据库 (CMDB),集成新的和以前的SSR数据.
主要成果:
- 在南瓜基因组中发现了54,526个SSRs,其中二核酸是最占主导地位的动机.
- 开发59个具有高多态信息含量 (PIC) 的多态SSR标记.
- 建立CMDB,为研究界提供获取验证的SSR.
结论:
- 这项研究为提供了宝贵的全基因组SSR资源.
- 开发的多态标记物对于DNA指纹,生殖质特性和分子繁殖至关重要.
- 该CMDB是推进果仁和相关物种遗传研究和育种的关键平台.
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