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全基因组对大豆冷沉浸应激的分析, Glycine max

Siwar Haidar^1,2, Simon Lackey^1,2, Martin Charette¹

¹Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, ON, Canada.

Frontiers in plant science

|September 6, 2023

概括

冷水对大豆发芽产生负面影响. 研究人员确定了三种定量特征基点 (QTLs) 对于冷水浸泡耐受性,有助于开发更耐用的大豆品种用于早期种植.

科学领域:

农业科学农业科学
植物遗传学植物遗传学
植物生理学作物生理学

背景情况:

加拿大农业由于短暂的无季节,需要提前种植作物.
早期种植的风险包括由于冷水浸泡 (<4°C) 而导致种子发芽的减少.
大豆 (Glycine max) 品种需要提高耐寒性,以获得可靠的早期播种.

研究的目的:

为了研究冷水浸泡对大豆种子发芽的影响.
为了确定与大豆冷水浸泡耐受性相关的遗传位置.
支持适合早期种植条件的大豆品种的发展.

主要方法:

对137种大豆品种在4°C和20°C的浸泡后的种子发芽的表型评估.
将一个三参数指数上升到最大方程 (3PERM) 中,以量化发芽参数.
基因组广泛关联研究 (GWAS) 使用基因型测序 (GBS) 衍生的SNP和R包 (rMVP,qvalue).

主要成果:

确定了五个与冷水浸泡后最大发芽相关的显著SNP.
在PVE和ASE分析后,染色体3,6和13上的三个定量特征位置 (QTL) 得到了确认.
通过基因本体学丰富,通过基因本体学丰富识别了14个参与发芽和耐寒通路的候选基因.

结论:

已识别的QTL提供了宝贵的遗传标记,用于培育具有增强冷水浸泡耐受性的大豆品种.
这些发现可以减轻与短生长季节的地区早期大豆种植相关的风险.
这项研究支持开发适用于不同农业环境的更有弹性的大豆品种.

关键词:

在GWAS中,GWAS就是GWAS.寒冷的压力是冷的压力.发芽发芽发芽是一个过程.这是一种沉浸式沉浸 (imbibition).豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆

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