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相关概念视频

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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相关实验视频

Updated: Jul 17, 2025

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
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Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix

Published on: December 22, 2011

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功能性成像指导的细胞选择,用于进化的遗传编码光指示器.

Chang Lin1, Lihao Liu1, Peng Zou1,2,3

  • 1College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing 100871, China.

Cell reports methods
|September 6, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了Faculae,这是一个新的成像平台,通过将细胞外观与遗传密码联系起来来设计光生物传感器. 这种方法快速选数千种变体,加速了先进的细胞成像工具的创建.

关键词:
指导进化是指导进化的.远红色的指标光成像成像技术的使用.基因编码的光指示器可光激活的细胞标记聚合选选 聚合选选 聚合选

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Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

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相关实验视频

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Real-time Live Imaging of T-cell Signaling Complex Formation
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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 细胞成像 细胞成像

背景情况:

  • 基因编码的光指标对于观察细胞动态至关重要.
  • 开发新的指标需要有效的方法来选大量变异.
  • 目前的方法在平衡选维度与吞吐量方面面临挑战.

研究的目的:

  • 介绍Faculae,一个功能性成像引导的可光激活细胞选择平台.
  • 为了使微观表型与基因型在聚合突变库中的直接链接.
  • 加快开发新的遗传编码光指示器.

主要方法:

  • 法库拉利用光激活在几秒钟内选择单细胞分辨率.
  • 该平台在哺乳动物细胞中同时评估数万种变异.
  • 它采用基于图像的聚合查方法.

主要成果:

  • 该研究成功地应用于远红色遗传编码指标 (FR-GECI) 的定向进化.
  • 该平台促进了指示器亮度 (Nier1b) 和信号与基线比率 (Nier1s) 的改进.
  • 证明了多维定向进化的可行性,用于生物传感器开发.

结论:

  • 面膜显著提高了遗传编码的光指标的发展.
  • 这种基于图像的聚合选方法对于创建各种生物分子工具具有多样性.
  • 该平台承诺加速细胞和分子生物学领域的创新.