相关实验视频

Updated: Jul 17, 2025

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Published on: June 17, 2022

在慢性骨髓性白血病治疗中,DNA自组装纳米花逆向P-糖蛋白介导的耐药性

Pengxuan Zhao¹, Yeteng Zhong², Pengcheng Pan¹

¹Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development of Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China.

Frontiers in bioengineering and biotechnology

|September 6, 2023

概括

研究人员开发了DNA纳米花来输送温克里斯 (VCR) 和P-gpsiRNA,在体外和体内有效治疗多药耐药慢性髓性白血病 (CML).

科学领域:

生物技术是生物技术.
纳米医学是一种纳米医学.
在瘤学瘤学.

背景情况:

慢性骨髓性白血病 (CML) 是一种由BCR-ABL氨酸激酶驱动的骨髓增殖性疾病.
芬克里斯 (VCR) 是一种标准的白血病治疗方法,但多药性耐药性 (MDR) 限制了其疗效.
在CML中,P-glycoprotein (P-gp) 是MDR的关键调解者.

研究的目的:

开发一种新的药物输送系统,以克服CML中MDR.
通过使用DNA纳米花来共同传递VCR和P-gpsiRNA.
评估该系统对抗耐药性CML的治疗潜力.

主要方法:

通过自我组装合成了DNA纳米花.
在DNA纳米花中装载了录像机和P-gp siRNA.
细胞毒性和治疗疗效在MDR CML细胞和体内模型中进行了评估.

主要成果:

DNA纳米花呈现出花形形状和高视频录像机加载效率 (80%).
纳米花成功地将VCR和P-gpsiRNA输入到MDRCML细胞中.
同时递送显著增强细胞毒性在体外和体内表现出强大的抗白血病活性,克服了MDR.

结论:

DNA纳米花代表了一个有前途的平台,用于共同交付VCR和P-gpsiRNA.
这种方法有效地克服了CML中的MDR,提供了一个新的治疗策略.
开发的纳米花系统显示了治疗耐药性CML的潜力.

关键词:

自组装的 DNA 自组装.慢性骨髓性白血病慢性骨髓性白血病药物输送是药物输送的过程.药物耐药性耐药性药物耐药性滚动圈放大放大滚动圈放大放大

更多相关视频

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Published on: January 7, 2019

相关概念视频

Treatment Resistant Cancers

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...

Combination Therapies and Personalized Medicine

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...