相关实验视频

Updated: Jul 17, 2025

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

RgnTX:在异构形异质性和模糊性存在的情况下,对转录组元素的局部化分析

Yue Wang^1,2, Zhen Wei^3,4, Jionglong Su⁵

¹Department of Mathematical Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu 215123, China.

Computational and structural biotechnology journal

|September 6, 2023

概括

RgnTX是一种新的R/生物导体工具,用于分析转录组元素,通过考虑转录组异质性和异构模糊性来解决现有方法的局限性. 它提供了一个灵活的方法来进行 colocalization 分析,产生明显和显著的结果.

科学领域:

生物信息学是一种生物信息学.
计算生物学计算生物学
基因组学就是基因组学.

背景情况:

对基因组区域的局部化分析对于理解功能相互作用至关重要.
现有的方法无法解释转录组异质性和异型模糊性.
由于复杂的拼接模式,转录组元素需要专门分析.

研究的目的:

开发RgnTX,一种R/生物导体工具,用于转录组元素的同位化分析.
通过结合转录组注释和灵活的零模型来解决现有方法的局限性.
为了分析缺乏明确的异形关联的转录组元素.

主要方法:

开发了RgnTX,这是一个R/生物导体包,使用了排列测试.
集成的转录组注释和灵活的零模型,以实现现实的背景模拟.
实现了可视化,z分数计算和多重假设测试 (本杰明-霍赫伯格) 的功能.

主要成果:

RgnTX有效地分析转录组元素,考虑到异质性和异构形态模两可.
在RgnTX中的新型测试模式比现有的基因组基因方法产生了不同的和更重要的结果.
使用合成和真实数据集验证RgnTX.

结论:

RgnTX 是一种有价值的工具,用于表征转录组随机性和执行统计关联分析.
该软件包增强了在异质转录组内对基因组区域集的分析.
对于研究人员来说,RgnTX可以通过生物导体免费获得.

关键词:

定居化分析的分析.异形模糊性是一种模糊性.测试多个假设测试.变试验变试验变试验有关RNA甲基化的RNA甲基化.转录组转录组就是一个转录组.

更多相关视频

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

相关概念视频

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...