数字粘膜样本中的结肠密室数量:用于诊断性结肠炎的新独立参数
Carlos A Rubio1, Corinna Lang-Schwarz2, Christian Matek2
1Department of Pathology, Karolinska Institute and University Hospital, Stockholm, Sweden.
Cancer diagnosis & prognosis
|September 6, 2023
概括
在结肠活检中计算交叉切割密码 (CCC) 可以区分活跃的炎症,如性结肠炎 (UC) 和传染性结肠炎 (IC),以及已解决的情况或正常组织. 这种可复制的方法有助于诊断结肠炎症.
科学领域:
- 胃肠病学 胃肠病学
- 病理学 病理学 病理学
- 数字病理学数字病理学
背景情况:
- 常规的结肠和直肠活检经常显示交叉切割密室 (CCC).
- 了解CCC分布对于准确的组织病理学诊断至关重要.
研究的目的:
- 在标准化数字结肠活检样本中量化交叉切割密码 (CCC).
- 评估CCC计数对区分各种结肠疾病的有用性.
主要方法:
- 研究了224个结肠活检,这些活检来自于患有性结肠炎 (UC),UC缓解 (UCR),感染性结肠炎 (IC),已缓解的IC (RIC),分离性西格炎 (DS) 和正常结肠粘膜 (NCM) 的患者.
- 分析了微观的同位数数字样本 (0.500毫米2) 来计算CCC.
- 利用标准化算法进行CCC计数,得到了三个欧洲国家的五位病理学家的验证.
主要成果:
- 在所有样本中,共计8024个CCC被计数.
- 与UCR,RIC,DS和正常结肠粘膜相比,在活跃的UC和IC中观察到明显较低的CCC频率 (p<0.05).
- CCC计数方法在病理学家中证明了可重复性.
结论:
- 在标准化的0.500毫米2数字结肠活检样本中计数CCC是一种可行的方法.
- 这种技术可以有效地区分长期的炎症 (UC,IC) 和持续的炎症或正常的粘膜 (UCR,RIC,DS,NCM).
- CCC计数算法是可重现的,有助于结肠炎症的差异诊断.
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