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计算流体动力学 纳米流体流体染色学中毛细血管误连引起的分散的研究
Ali Moussa1, Sander Deridder1, Ken Broeckhoven1
1Department of Chemical Engineering, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
Analytical chemistry
|September 6, 2023
概括
纳米流液体色谱 (LC) 的连接错误会影响分离效率. 虽然毛细管 misalignment 有最小的影响,毛细管间的差距显著增加分散,特别是更大的差距. 谨慎的连接设计对于高速LC至关重要.
科学领域:
- 分析化学 分析化学
- 分离科学 分离科学
- 流体动力学 流体动力学
背景情况:
- 在纳米流液体色谱 (LC) 中,高速和高效分离对连接质量非常敏感.
- 仪器连接对LC系统的分离性能具有重要影响.
研究的目的:
- 研究两种常见的连接错误 - - 毛细管错位和毛细管间隙 - - 对纳米流LC中的分散的影响.
- 量化间隙宽度和长度对LC连接中的分散的影响.
主要方法:
- 使用了计算流体动力学 (CFD) 模拟.
- 模拟了两种类型的连接错误:毛细血管错位和毛细血管间隙.
- 分析了间隙半径宽度 (Dc) 和长度 (Lc) 对分散的影响.
主要成果:
- 显著的毛细管 misalignment (高达75%的相对误差) 并没有引入实质性的分散.
- 毛细管间隙显著增加了分散,随着间隙辐射宽度 (Dc) 的快速增加,差异性迅速增加.
- 分散损失在Dc ≤ 30μm和Lc ≤ 200μm的1.5μL/分钟流量时仅限于~1nL2.
结论:
- 在纳米-LC连接中,毛细管 misalignment 容忍度高于预期.
- 毛细管间隙是分散的主要来源,需要狭窄的孔隙和精确的对齐.
- 如果毛细血管完美匹配,零死体积连接器可以有效,最大限度地减少纳米LC和微流体系统中的分散.
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