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一种基于细胞杂交的方法,用于产生用于检测细胞因子的重组子单克隆抗体

Yu Peng1, Chun'e Zhang1, Minyan Deng1

  • 1Bio-Rad (Shanghai) Life Science Research and Development Co., Ltd, Shanghai, China.

BioTechniques
|September 6, 2023
PubMed
概括

产生复合子单克隆抗体 (子rAbs) 现在更容易,更便宜. 这种新方法可以产生用于各种研究应用的高 afinity 子 rAbs.

关键词:
艾丽莎·伊丽莎·伊丽莎亲和关系是一种亲和关系.细胞杂交的细胞杂交.子复合抗体的复合抗体表面的等离子体共振.

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Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Commonly used fusion techniques — electroporation,...
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