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莫纳斯克的多重代谢途径工程增强了洛瓦斯的生产

Xiaokun Hong¹, Tianlong Guo¹, Xinqi Xu²

¹College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350116, Fujian, China.

Applied microbiology and biotechnology

|September 6, 2023

概括

莫纳斯克 (Monascus pilosus) 的代谢工程去除了真菌毒素 (mycotoxin) 氨酸,并增加了洛瓦斯塔丁的产生. 这为食品应用创造了更安全的菌株,增强了其作为降胆固醇剂的潜力.

科学领域:

食品微生物学食品微生物学
代谢工程是代谢工程.
工业生物技术工业生物技术

背景情况:

莫纳斯库斯物种是有价值的食物微生物,生产降胆固醇剂洛瓦斯塔丁.
然而,毒素氨酸的存在和低洛瓦斯塔丁产量限制了它们在食品工业中的应用.
代谢工程提供了一条克服这些局限性的途径.

研究的目的:

为了基因修改Monascus pilosus GN-01以消除氨酸的生产.
为了提高修改后的M. pilosus菌株的洛瓦斯塔丁产量.
为食品应用开发一种安全且高产的菌株.

主要方法:

破坏氨酸合成调节基因 (ctnR) 以产生无氨酸菌株 (GN-02).
洛瓦斯塔丁生物合成基因 (mokC, mokD, mokE, mokF, mokH, mokI 和 LaeA) 的过度表达.
敲除色素调节基因 (pigR) 以进一步促进洛瓦斯塔丁的产生.

主要成果:

ctnR基因的破坏成功地抑制了M. pilosus GN-01.1中的氨酸生产.
超表达的洛瓦斯塔丁生物合成基因和猪R淘汰显著增加了洛瓦斯塔丁含量.
与没有氨酸的菌株 (GN-02) 相比,pigR淘汰赛菌株的洛瓦斯塔丁产量增加了76.60%.

结论:

多重代谢通路工程是有效的改善洛瓦斯塔丁的生产在蒙纳斯库斯.
开发的没有氨酸的M. pilosus菌株为高洛瓦斯塔丁产量提供了安全有效的选择.
这种工程菌株有望在与Monascus相关的食品中应用.

关键词:

基因淘汰赛基因淘汰赛基因淘汰赛过度表达的基因表达过度洛瓦斯塔丁是什么意思洛瓦斯塔丁是什么意思莫纳斯克毛虫 (Monascus pilosus) 是一种毛虫.

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