赛尔调节牛胚胎的发育动力学和转录组
Kelsey Nicole Lockhart1, Jessica N Drum1, Ahmed Z Balboula1
1Division of Animal Sciences, University of Missouri, Columbia, Missouri, USA.
概括
男性生育能力显著影响牛的早期胚胎发育. 性能较低的母产生的胚胎具有较高的细胞压力,导致在怀孕的关键第一周发育停止.
科学领域:
- 生殖生物学 生殖生物学
- 胚胎学 胚胎学
- 动物科学动物科学
背景情况:
- 在牛群中,怀孕早期的流产在怀孕的第一个星期是常见的.
- sire 对植入前胚胎发育的影响还不太清楚.
研究的目的:
- 为了研究 sire 对牛早期胚胎发育的影响.
- 为了比较来自高性能 (HP) 和低性能 (LP) 胚胎的胚胎.
主要方法:
- 在体外生产牛胚胎,使用来自HP和LP母体的雌性体.
- 监测胚胎发育和确定停产阶段.
- 对早期胚胎的自活动和基因表达的分析.
主要成果:
- 五到六细胞阶段是胚胎最常见的停留点.
- 来自LP sires的胚胎与HP sires的胚胎相比显示出自活动的增加.
- 来自LP的胚胎表现出精子线粒体清除,基因素保留和DNA损伤的潜在问题.
结论:
- 赛尔的遗传贡献显著影响了早期胚胎发育和生存.
- 低效率的乳母会增加牛胚胎的细胞应激和发育停滞.
- 了解生长效应对于提高牛的生殖效率至关重要.
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