通过代谢工程和生物工艺开发,Pseudomonas putida将宁转化为β-ketoadipic酸
Allison Z Werner1, William T Cordell1,2, Ciaran W Lahive1
1Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.
Science advances
|September 6, 2023
概括
工程微生物将红素化合物转化为β-基酸,一种聚合物前体. 这种生物工艺实现了高产量和具有成本竞争力的定价,为化石燃料提供了可持续的替代品.
科学领域:
- 生物技术和代谢工程 生物技术和代谢工程
- 可持续化学 可持续化学
- 生物精炼是生物精炼的一种方式.
背景情况:
- 由于其复杂的结构,质素的价值化存在挑战.
- 微生物生物催化提供了一种可持续的途径,将素衍生的芳香化合物 (LRC) 转化为有价值的产品.
- β-基托酸是高性能聚合物的关键前体.
研究的目的:
- 为了工程师 * 伪虫 putida * KT2440 的混合 LRCs 的有效转换到β-ketoadipic 酸.
- 优化酶表达和全球调节,以提高产品标位和生产率.
- 评估生物工艺的经济可行性,用于树脂素的价值化.
主要方法:
- 基因工程的 * 伪白 * KT2440.
- 调整参与芳香化合物代谢的关键酶的表达 (O-脱甲基,基,环开放).
- 删除一个全球调节器,以增强对β-基托阿迪皮酸的代谢流.
- 对生物工艺和下游净化技术经济分析.
主要成果:
- 从模型LRC中获得的β-基托阿迪皮酸标位为44.5g/L,从玉米炉中获得的LRC中获得的LRC为25g/L.
- 获得的生产率为1.15g/L/h (模型LRC) 和0.66g/L/h (来自玉米炉的LRC).
- 显示的总产量为0.10g的β-基托阿迪皮酸每克玉米炉衍生的红素.
- 预计β-基托阿迪皮酸的最低销售价格为2.01美元/千克,与化石来源的阿迪皮酸竞争.
结论:
- 成功设计了一种微生物菌株,以有效地将混合的LRC转化为β-基托阿迪皮酸.
- 生物工艺证明了将红素价值化为性能优化的生物产品的经济可行性.
- 这项工作促进了从可再生木质素资源中可持续生产聚合物前体的发展.
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