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Updated: Jun 24, 2026

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

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免疫亲和性LC-MS/MS 通过使用磁性和聚乙烯珠固定多克隆抗体量化败血症生物标志物普罗卡西托尼

Sebastian-Alexander Tölke1,2, Thomas Masetto3,4, Thomas Reuschel1

  • 1Institute for Biomolecular Research, Hochschule Fresenius, University of Applied Sciences, Limburger Straße 2, 65510 Idstein, Germany.

Journal of proteome research
|September 6, 2023
PubMed
概括

准确量化 procalcitonin (PCT) 对于败血症诊断至关重要. 这项研究开发了一种敏感的LC-MS/MS方法,以协调临床试验并改善细菌败血症检测.

关键词:
在 LC-MS/MS 量化中.这是一个PCTPCT.免疫强化 免疫强化这是什么意思? procalcitonin.标准化 标准化 标准化

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Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...