尽管发生了起始代码突变,但Mycobacterium bovis BCG多德基因编码了一个功能性蛋白质
Marcos Gustavo Araujo Schwarz1, Paloma Rezende Correa1, Paula Silva Lacerda Almeida1
1Laboratório de Genômica Funcional e Bioinformática, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil.
Tuberculosis (Edinburgh, Scotland)
|September 6, 2023
概括
卷入flavin稳态的mycobacterium tuberculosis dodecin是由BCG中的ATG和ACG起始码子生成的. 这种蛋白质赋予氧化应激抵抗力,表明其作为结核病药物点的潜力.
科学领域:
- 微生物学 微生物学
- 蛋白质生物化学 蛋白质生物化学
- 结核病研究 结核病研究
背景情况:
- 多德是一种多德体,对Mycobacterium tuberculosis中的黄素稳态至关重要,具有显著的温度和度耐受性.
- 预计Bacillus Calmette-Guérin (BCG) (ATG到ACG) 中的多德基因起始编码子中的单核酸多态 (SNP) 会产生一个短的N端异型,缺少七种氨基酸,具有减少的极端友好性质.
研究的目的:
- 在真菌细菌环境中,从ATG和ACG等位基中研究活体生产多德.
- 为了评估二氧化应激反应中从两个等位基生成的多德的功能影响.
主要方法:
- 使用mCherry克隆的下游和内框架与M.结核病 (M.tb) 和BCG多德上游区域进行报告者基因分析.
- 在Mycobacterium smegmatis中进行补充测试,以评估在氧化应激 (过氧化暴露) 下增长的增强.
主要成果:
- 报告者基因测定证实了从M.tb和BCG等位基因中产生功能性多德的产生.
- 补充研究表明,这两种多德基因基因都类似地增强了M. smegmatis在后对数阶段和过氧化暴露期间的生长.
- 这些发现表明多德在氧化应激反应机制中的作用.
结论:
- 尽管SNP改变了起始编码子,但BCG编码子的产生,尽管水平低于M.结核病编码子,尽管SNP改变了起始编码子.
- 从BCG等位基产生的功能性多德赋予了与M.tb等位基产生相似的表型.
- 多德是开发抗结核新疗法的潜在药物标.
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