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在人类皮层发育过程中,LIF信号调节外辐射质细胞与内核神经元命运

Madeline G Andrews¹, Clara Siebert², Li Wang²

¹Department of Neurology, University of California, San Francisco (UCSF), San Francisco, CA 94143, USA; The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, CA 94143, USA; School of Biological and Health Systems Engineering, Arizona State University (ASU), Tempe, AZ 85281, USA.

|September 6, 2023

概括

白血病抑制因子 (LIF) 信号影响外部辐射质细胞 (oRG) 的发展. LIF促进ORG细胞产生抑制性内部神经元 (INs),影响大脑皮层组织.

科学领域:

神经科学是一个神经科学.
发展生物学发展生物学
干细胞生物学干细胞生物学

背景情况:

放射性质细胞 (RG) 的发育对于大脑皮层的生长至关重要.
外部放射性质细胞 (oRG) 亚型在人类中被扩展,产生多样化的神经元和质细胞.
调节ORG分化的机制在很大程度上是未知的.

研究的目的:

研究白血病抑制因子 (LIF) 在ORG细胞分化中的作用.
为了确定oRG细胞是否能够产生抑制性内部神经元 (INs).
阐明控制ORG细胞命运的信号通路.

主要方法:

在神经发生过程中对ORG细胞中LIF受体表达的分析.
在皮质组织和有机体中LIF信号的扰乱.
对IN种群的比较转录基因分析.
通过LIF治疗分离和培养初级oRG细胞.

主要成果:

在皮层模型中,LIF信号影响ORG扩散.
LIF治疗增加了来自oRG细胞的抑制性内部神经元 (IN) 的产生.
由LIF诱导的INs类似于源自尾部质突起的INs.

结论:

在ORG的发展中,LIF信号发挥着双重作用,影响了核武的扩散和核武产量.
oRG细胞具有产生IN的能力,这是一种以前被低估的发育途径.
LIF作为oRG细胞命运的关键调节者,指导它们的分化向IN系.

关键词:

大脑皮层大脑皮层人类发展人类发展这是一种抑制性内部神经元.差异化机制的差异化机制神经原始体的神经祖先神经发育的神经发育有机体模型有机体模型多能干细胞是一种多能干细胞.辐射性质细胞信号机制的信号机制.

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