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对于微生物电合成的Shewanella oneidensis的代谢调节:从细胞外到细胞内

Yixin Li¹, Qingliu Luo², Jiaying Su³

¹Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, 361005, China.

Metabolic engineering

|September 6, 2023

概括

通过对细胞外电子吸收和二氧化碳转化通路进行基因改造,Shewanella oneidensis MR-1微生物电合成 (MES) 的性能得到了提高. 关键基因的共同过度表达显著提高了形式的产生,提高了MES的效率.

科学领域:

微生物学微生物学
生物技术是生物技术.
合成生物学合成生物学

背景情况:

谢瓦尼拉 (Shewanella oneidensis) MR-1 在微生物电合成 (MES) 中表现出高效率.
优化电子转移和二氧化碳转化对于提高MES性能至关重要.

研究的目的:

研究和调节涉及S. oneidensis MR-1细胞外电子吸收 (EEU) 和细胞内二氧化碳转化中的基因,以改善MES.
阐明在MES中用于格式合成的电子利用机制.

主要方法:

基因工程S.oneidensis MR-1在EEU中的关键基因过度表达 (mtrB,mtrC,mtrD,mtrE,omcA,cctA) 和二氧化碳转化 (nadE,nadD,nadR,nadV,pncC,petC).
甲酸脱酶基因 (fdhA1,fdhB1,fdhX1) 的过度表达.
选择基因 (cctA,fdhA1,nadV) 的联合过度表达,以评估对形式生产力的联合影响.

主要成果:

过度表达EEU基因增加了1.5-2.1倍的形式生产力.
过度表达二氧化碳转化基因增加了1.3-3.4倍的形式生产力.
甲酸盐脱酶基因的过度表达使甲酸盐的生产率增加了2.3-3.1倍.
对cctA,fdhA1和nadV的共同过度表达导致格式生产率增加了5.59倍.
确定了涉及ATP,NADH和CO2的电子利用途径,用于形式合成.

结论:

对S. oneidensisMR-1的基因改造显著提高了微生物电合成性能.
针对EEU和CO2转换途径提供了一种可行的策略,用于改善MES中的格式生产.
这项研究为MES中的电子流和格式合成提供了机械的见解,为进一步的遗传优化铺平了道路.

关键词:

细胞外的电子吸收.细胞内二氧化碳的转化.微生物的电合成.这种植物是Shewanella oneidensis.

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