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在甲基胺诱导的识别记忆缺陷中,周围回路到前额回路

Jordan L Hopkins¹, Sarah T Goldsmith¹, Samuel K Wood¹

¹Reichel Laboratory, Medical University of South Carolina, Department of Neuroscience, Charleston, SC, 29425, USA.

Neuropharmacology

|September 6, 2023

概括

长期使用甲基胺会损害记忆. 这项研究揭示了从周围皮质 (PRH) 到中部前额皮质 (mPFC) 的通信对于对象识别记忆至关重要,激活这个电路可以扭转甲基因诱导的缺陷.

科学领域:

神经科学是一个神经科学.
成研究研究成研究
认知心理学认知心理学

背景情况:

长期使用甲基胺 (meth) 会导致认知缺陷和皮质功能障碍.
长期在老鼠中自行服用甲基会导致对象识别记忆障碍,这与皮质周围膜皮层 (PRH) 的可塑性有关.
PRH具有广泛的投影,包括中枢前额叶皮层 (mPFC),这表明这个电路在记忆中可能发挥作用.

研究的目的:

研究PRH-mPFC互换电路在新型物体识别内存中的作用.
在记忆任务期间,确定PRH-mPFC电路内的信息流的方向性.
评估操纵PRH-mPFC电路活动对甲基诱导的记忆缺陷的影响.

主要方法:

编码GFP标记的Cre复合酶的病毒载体 (rgAAV) 被注入大鼠的PRH或mPFC中.
鼠被测试在新的物体识别任务上,探索熟悉和新鲜的物体,或仅仅是熟悉的物体.
在mPFC和PRH中分析了GFP和Fos表达;使用化学遗传技术来抑制或激活PRH-mPFC电路.

主要成果:

投射到mPFC的PRH神经元被新型物体不成比例地激活,这表明PRH到mPFC的通信流.
在PRH中,Fos阳性细胞的百分比与识别记忆性能正相关.
化学遗传抑制PRH-mPFC电路损害了对象识别记忆,而激活则在以前依赖的老鼠中逆转了甲基诱导的缺陷.

结论:

从PRH到mPFC的通路对于对象识别内存至关重要.
激活这个电路可以改善与慢性甲基胺使用相关的记忆障碍.
这些发现突出了比以前理解的更复杂的神经电路,是识别记忆的基础.

关键词:

禁欲禁欲禁欲禁欲禁欲吸毒成吸毒成是一种成.认知功能障碍认知功能障碍脑内内皮层 (entorhinal cortex) 是一个内脑皮层.记忆记忆记忆记忆记忆药物使用障碍药物使用障碍

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