相关实验视频

Updated: Jul 17, 2025

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Published on: August 2, 2012

通过Pyrene Excimer形成探测到低代PAMAM树突体中的内微分子形状变化

Sanjay Patel¹, Stuart A McNelles², Alex Adronov²

¹Institute for Polymer Research, Waterloo Institute for Nanotechnology, Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L 3G1, Canada.

The journal of physical chemistry. B

|September 6, 2023

概括

二烯催化体形成 (PEF) 揭示了二烯标记的树枝状体的结构变化. 添加酸会导致树枝膜膨胀,这是PEF可以检测到的可逆变化.

科学领域:

聚合物化学聚合物化学
超分子化学超分子化学
材料科学材料科学材料科学

背景情况:

体是高度分支的大分子,具有独特的特性.
了解树枝状分子的结构变化对于它们的应用至关重要.
皮尔尼埃克西默形成 (PEF) 是对局部环境的敏感探针.

研究的目的:

为了研究烯标记的PAMAM树状体中的内微分子构造变化.
使用PEF量化pH值对树枝状分子结构的影响.
为了证明PEF作为研究宏分子构造的工具.

主要方法:

合成标有烯的PAMAM树突体 (PyCX-PAMAM-GY).
时间分辨率光光谱法测量烯单体和异构体衰变.
无模型分析 (MFA) 用于确定除菌体形成速率常数.
与局部烯度和扩散控制速率常数进行比较.

主要成果:

在添加HCl时,观察到排泄物形成速率常数 (k) 的下降.
这种下降归因于由内部氨基酸的质子化引起的树突膜胀.
PEF测量表明,在质突发生后,树突半径可逆增加了13%.
使用模型化合物将溶剂效应 (DMF与DMSO) 规范化.

结论:

PEF是一种强大的技术,用于定量检测宏分子构造变化.
在PAMAM树状体中内部三级胺的质子化导致显著的胀.
形状变化是可逆的,可以通过PEF监测.
PEF补充了其他技术,如用于结构分析的散射.

更多相关视频

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Published on: February 18, 2014

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

相关概念视频

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...